nmba chemical  (Ash Stevens Inc)

Journal: Molecules

Article Title: Determination of Genotoxic Impurity N -Nitroso- N -methyl-4-aminobutyric Acid in Four Sartan Substances through Using Liquid Chromatography–Tandem Mass Spectrometry

doi: 10.3390/molecules27217498

Figure Lengend Snippet: HPLC chromatograms of NDMA-d 6 , NMBA and four kinds of sartan substances on the C18 column.

Article Snippet: NMBA (purity ≥ 98.93%) and NDMA-d 6 (purity ≥ 99.50%) were purchased from Cato Research Chemicals Inc. (Guangzhou, China).

Techniques:

Journal:

Article Title: Effects of Phenylethyl Isothiocyanate on Early Molecular Events in N -Nitrosomethylbenzylamine-Induced Cytotoxicity in Rat Esophagus

doi: 10.1158/0008-5472.CAN-06-4531

Figure Lengend Snippet: Effect of PEITC on NMBA-induced preneoplastic lesions in rat esophagus. Microscopic esophageal tissue sections (magnification ×100) stained with H&E. A, a, vehicle control; b, PEITC treated; c, NMBA treated (note extensive cytotoxicity and inflammatory response); d, NMBA + PEITC treated. B, percent of normal, hyperplasia, and low-grade dysplasia area in vehicle control, NMBA, PEITC, and NMBA + PEITC–treated esophagus.

Article Snippet: Chemicals NMBA was purchased from Ash Stevens, Inc., and PEITC was from Aldrich Chemical Company.

Techniques: Staining, Control

Journal:

Article Title: Effects of Phenylethyl Isothiocyanate on Early Molecular Events in N -Nitrosomethylbenzylamine-Induced Cytotoxicity in Rat Esophagus

doi: 10.1158/0008-5472.CAN-06-4531

Figure Lengend Snippet: PEITC modulates NMBA-induced changes in gene expression. A, gene expression profiles for 1,323 genes that are differentially expressed in response to NMBA treatment and also differentially expressed comparing NMBA to PEITC + NMBA treatment. B, data points reflect the mean value for three replicate arrays. Y axis, normalized gene expression levels in which the expression value for each treatment of a gene was divided by the average of all treatments for that gene. C, to provide a comparison of genome-wide expression changes for different treatments, we applied principal components analysis to all of the 12 microarrays used in the study. Each of the 12 microarrays used were clustered with the axes representing the first three principal components of gene expression, based on the expression of all 41,070 probes on the arrays. NMBA-treated samples () are distinct in global gene expression whereas those samples treated with PEITC alone () or cotreated with PEITC + NMBA () are clustered near the vehicle controls ().

Article Snippet: Chemicals NMBA was purchased from Ash Stevens, Inc., and PEITC was from Aldrich Chemical Company.

Techniques: Gene Expression, Expressing, Comparison, Genome Wide

Journal:

Article Title: Effects of Phenylethyl Isothiocyanate on Early Molecular Events in N -Nitrosomethylbenzylamine-Induced Cytotoxicity in Rat Esophagus

doi: 10.1158/0008-5472.CAN-06-4531

Figure Lengend Snippet: Differentially expressed genes in the esophagus of rats treated with NMBA, PEITC, or NMBA + PEITC and associated with phase I and phase II metabolizing enzymes “1”

Article Snippet: Chemicals NMBA was purchased from Ash Stevens, Inc., and PEITC was from Aldrich Chemical Company.

Techniques: Sequencing

Journal:

Article Title: Effects of Phenylethyl Isothiocyanate on Early Molecular Events in N -Nitrosomethylbenzylamine-Induced Cytotoxicity in Rat Esophagus

doi: 10.1158/0008-5472.CAN-06-4531

Figure Lengend Snippet: Biological processes in NMBA-treated rat esophagus that were modulated by PEITC treatment

Article Snippet: Chemicals NMBA was purchased from Ash Stevens, Inc., and PEITC was from Aldrich Chemical Company.

Techniques:

Journal:

Article Title: Effects of Phenylethyl Isothiocyanate on Early Molecular Events in N -Nitrosomethylbenzylamine-Induced Cytotoxicity in Rat Esophagus

doi: 10.1158/0008-5472.CAN-06-4531

Figure Lengend Snippet: RT-PCR validation of microarray results. Gene expression was measured in selected genes, Gst1a2, Pcna, and Mapk6 using RT-PCR. Each sample was assayed in triplicate for each gene. Each sample represents pooled RNA created from equal amounts of total RNA from three independent RNA samples, representing individual animals. Each of these samples was obtained from a different animal, and no sample was included in more than one pool. In total, samples from nine animals per group were pooled for control, NMBA, PEITC, and PEITC + NMBA. In all cases, RT-PCR measurements (vertical filled bars) confirmed the changes in gene expression identified by microarray analysis (dotted filled bars).

Article Snippet: Chemicals NMBA was purchased from Ash Stevens, Inc., and PEITC was from Aldrich Chemical Company.

Techniques: Reverse Transcription Polymerase Chain Reaction, Biomarker Discovery, Microarray, Gene Expression, Control